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steroidal sapogenins separation
Posted: Thu Feb 05, 2009 7:45 pm
by vestelshirley
I have been trying c18 column with meoh/water (80:20) and some other similar mobile phases with no luck on separating the steroidal sapogenins extracted from a nut. I have a reference by Hunter and another by JT Lin where they use Acetonitrile/Hexane/THF but no relative amounts are listed. I need to separate about 4 or 5 compounds that closely elute with the meoh/h2o mobile phase. Might anyone have a clue as to what relative amounts of the above-mentioned cocktail are?
Posted: Sat Feb 07, 2009 1:31 am
by Uwe Neue
I have no clue about the relative amounts. On the other hand, if you are looking also for suggestions on a better separation method, I would throw some THF into the mobile phase or even completely replace the MeOH. You will definitely get a different elution pattern.
sap sep
Posted: Mon Feb 23, 2009 1:30 pm
by vestelshirley
Uwe,
Thanks for your reply. I tried methanol/water 40/60 and had several c18 columns on hand, so I linked three in series 450 mm and ran a 0.2 mL/min flow and got ok separation.