Chromatography Forum

Here are some websites for micro-boar applications:

http://www.hitsusa.com/1-good-ones/tetl ... up-pig.jpg
http://www.teacuppiggies.com/

I would try the micro-boar methods, there can be some serious volatility issues with the mega-boars.

I just learned that one can buy pleasure regulators. However, I am trying to use ultra-high pleasure techniques to separate my pigs. So I do not think that a pleasure regulator would be useful. Any opinions?

On must be careful! After all, if a fitting bursts, there may be a loss of pigs and pleasure.

You may not be looking at the proper sources for an ultra-high pleasure regulator. For instance, I would suspect that you could find these in Las Vegas, however we are all aware of the special interstate commerce rules for Las Vegas, namely what happens in Vegas stays in Vegas. So even though there may be ultra-high pleasure regulators in Las Vegas you have to use them locally, and can't remove them from the city.

There are also foreign cities such as Amsterdam, and foreign countries such as Thailand where I believe that it is not that difficult to find ultra-high pleasure regulators. Here there will probably be customs issues with export.

Are the pigs up for a road trip? Las Vegas is probably easiest, it is much easier to transport livestock across state lines than over borders, but there is still paperwork to be done. In Vegas there is also the possibility of random loss of pigs to the buffets.

@Don_Hilton: I disagree - there may be an increased risk of pigs in the case of a burst pleasure regulator...

A pleasure regulator - especially an ultra high pleasure regulator - doesn't sound to me like something one might find in Vegas or Amsterdam or Thailand because they're all about such things being somewhat unregulated, no?

I know that I wouldn't want my (pigs) pleasure, ultra high or not, to be regulated...it seems that it would put something of a damper on it.

Thanks to juddc and to everybody else for your inputs! I now agree that it is best to maximize the pleasure to maximize the pig production rate. I also think that the theoreticians in this field will agree with this thought.

The pigs are hungry and need to be fed...

The pigs are hungry and need to be fed...

I'll add a bucket of slop to the discussion.

Let's review the possibilities for solving our pig separation/detection problem.

Gas chromatography can be immediately excluded since pigs are non-volatile, although FID (Flavorable Ionization Detection) would work well for detection.

Size exclusion separation Should work if trying to separate pigs according to size.

Ion-exchange separation Pigs must be charged or ionized. Charging pigs are difficult to work with.

Normal-phase separation Works well with polar bears. Not sure how well it will work with pigs due to the differences in the sample environment.

Reverse-phase separation Pigs are asymetrical in structure but not sure if they have polarity. May need to add some ion-pairing agent.

Refractive index detection Should work since pigs have a large refractive index.

UV\visible detection Should also work since pigs absorb at all wavelengths.

Fluorescence detection Don't know about this one. Have not consumed enough of the lab alcohol to see visions of fluorescent pigs.

Let the discussion continue

There are also foreign cities such as Amsterdam, and foreign countries such as Thailand where I believe that it is not that difficult to find ultra-high pleasure regulators. Here there will probably be customs issues with export.

I would watch out for the fittings in Thailand though. Many people, expecting M - F connectivity, have inadvertantly found themselves with M - M fittings causing acute embarrasment

Size exclusion has been discussed before, and there were some good suggestions.

I do not understand what one can do with normal-phase techniques. I understand the mechanism that is active for polar bears, but I am not aware of any polar pigs. Anyway, I don't think that my pigs are polar enough.

Yes, paired ion reagents (PIG reagents) will work. I will look into that.

Experts have told me that the tail of the pigs can interfere with quantitation. Shall I cut off the tails of all my pigs?

[quote="skunked_onceFluorescence detection Don't know about this one. Have not consumed enough of the lab alcohol to see visions of fluorescent pigs.

quote]

http://www3.interscience.wiley.com/jour ... 1&SRETRY=0

http://www.msnbc.msn.com/id/22563650/

Peter

Google helps.
" PIG separation" found, as first hit...

http://www.aseansources.com/en/JES0012/product/Pigging

I assume you have a family of Plain Pigs, whereas you require Flexi-separation or Separation Pigs.

It's mid-winter here, so pig separation based on hydrophobic interaction is possible with fields of mud coated with a porkshell of green carboydrate fibres. Small pigs will not sink so deep as large pigs when they run to the slop trough. The separation is not chiral, so chopping curly tails off may be needed.

A calibrated acoustic emission (Oink) detector will locate the pigs without destruction, but a moving wire detector might produce barbequed pork, if required.

Experts have told me that the tail of the pigs can interfere with quantitation. Shall I cut off the tails of all my pigs?

In your case, the tail will be making a negligable contribution to the overall pig size (whether you measure pig area or pig height). Therefore you can consider the contribution of the pig tailing to be beneath the level of accuracy of the separation and quatification method you choose.

In fact, with size exclusion methods the pig tail is springy enough not to affect the separation at all, it will merely bend out of the way as the pig walks under the bar.