HPLC Method for sodium acetate anydrous determination

[alemaggot]

Today I've found a GC method that use BSTFA [bis(trimethylsilyl)trifluoroacetamide] as derivatization solvent. There's write that I can't use Methanol because he coeluite with acetic acid.
Also there's write that is better to don't use water as solvent. There's write to use acetonitrile a solvent.

It's a valid method?

[DSP007]

Before you try sodium acetate you need to test whether or not you can see acetic acid when that is the only component in the sample.

Make up a 1% solution of acetic acid, and adjust you headspacer and GC settings until you can see a nice sharp peak that is not present when you analyse clean water. The halve the concentration of acetic acid and see whether the peak gets smaller. If it does it is probably acetic acid.

From the amounts of sodium acetate that you used it seems that the concentrations that you are expecting in your samples might be rather low. Can you confirm what concentrations you are expecting ?

Peter

Hi Peter!

In my product the sodium acetate limit is less than 0,02%. Usually there is a small residue of it after production (about 0,003 %)

Well, you give ....
Are these accidents 0.003% as can be some affect on the biological activity or stability of the product?

[alemaggot]

I don't think it DSP.

However what you think about derivatization method? If you can look on my other post "costant decrease area". I've write a problem that I happened yesterday during the try with acetic acid.

Thanks!