Chromatography Forum

Pork loin, marinated in lime and coriander and then onto the BBQ.

No, no guys Uwe wanted a separation method, now you are talking about post column derivatizations that are not even likely to be specific to micropigs as a class, let alone to specific individual micropigs.

Bisnettrj2's picture clearly shows the effect of temperature on pig separation - running everything at around 30C will get them more widely separated, but if your mobile phase flow rate is too high at those temperatures your pigs will suffer a thermal degradation that will immobilise them onto the substrate.

Peter

May not be the original project, but the discussion has taken my mind off in the direction of a recipee for ribs using chili sauce, garlic, five spice powder - and I'll have to look up the rest.

I fear that constraining the discussion will be like hearding the cats mentioned above...

Don, your mention of herding just gave me an idea. The ideal separation device might be a border collie. They have a very strong herding instinct, are easily trained, and would be willing and able to separate pigs as directed by the handler if properly trained.

Note - in addition to providing only a partial separation based on size exclusion, we need to be reminded that gates don't work on all classes of pig...

From personal experience with livestock, farm animals prefer to squeeze through a small gap they are not supposed to go through in preference to going through a large gap they are supposed to. I would say that active sorting would be more effective than passive sorting (e.g. passing through a series of gates with no guidance), normal separation techniques we use in the lab are based on separating inanimate molecules, not living, intelligent animals. Of course, some animals are more equal than others, pigs and mules are quite intelligent, while your average sheep is about as smart as a rock.

Ok, to guide the pigs 'actively' through the various gates, a mild electric shock can be appied to the floor of my pen (proposed previously) in a stepwise fashion, starting at the largest gates, and proceeding to the smallest, thus giving direction (and impetus) to the separation.

Electricity, Pigs, Barbecue sacue...

I think I need to get away from this computer and get some supper.....

I think we made them upset:
http://www.zooborns.com/.a/6a010535647b ... 8ae970b-pi

Yikes. Looks like someone crossed a pig with a Sharpei and smacked the result with an ugly stick. Uwe, are you sure you want to separate these 'things' from "Snow White"?

I think we made them upset:
http://www.zooborns.com/.a/6a010535647b ... 8ae970b-pi

Auntie Em warned you what would happen if the pigs weren't fed on time... (from the Wizard of Oz)

Pigs are routinely and easily separated using the PigKit (c) derivatisation package from MegaPigTechnology together with a SupaStrong Crosslinked PigXtra gate. It's the only gate out there that's guaranteed to provide separation down to the nearest inch, with a theoretical bar-count of at least 500. A complete separation can be achieved in under 3 minutes per decaPig, and it's fully compatible with ultra-high-pressure pig-handling systems. I can send you application notes. Just contact your MegaPigTechnology rep...

(...who, secretly, is me, but I'm not admitting that in my signature; let the flame-wars begin between me and the SwineSep rep)

I think you first need to write a cGMPig protocol.

Experts told us that we should measure the pigs' plates, but we could not find any...

Experts told us that we should measure the pigs' plates, but we could not find any...

In chromatography terms, the pigs are applied to the plates.