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Posted: Thu Dec 18, 2008 12:12 pm
by carras
Stryder08, The LOQ is 0.1 ng/ml in urine. At this concentration the EU requirements for confirmation are met (see last post by Jerole).
Best Regards
Posted: Fri Dec 19, 2008 9:52 am
by jerole
Two thougths: I am assuming that you've tried the procedure with a standard, if that's the case be sure that you have evaporated completely the solvent you've pipeted into the derivatitation vial.
That might be it, we will try again after evaporating the solvent.
By the way, are you from Spain?
Posted: Sat Dec 20, 2008 8:46 am
by carras
That`s right, from Madrid.
Posted: Sat Dec 20, 2008 1:33 pm
by jerole
That`s right, from Madrid.
That's great! I'm from Oviedo. Maybe I could call you if we have still trouble with the reaction?
Posted: Sun Dec 21, 2008 7:54 am
by carras
Sure, no problem.
Posted: Mon Dec 29, 2008 9:13 pm
by gpronger
Has the use of a capillary direct injection been evaluated without derivitization? Looking at its structure, if the hot inlet was avoided, the compound may be sufficiently stable.
Posted: Tue Dec 30, 2008 7:34 am
by carras
I have never tried it and I guess it doesn't work well since all bibliography on GC/MS for this compound uses some kind of derivatitation. Anyway, the big problem is unstability in the ion source of the trimethylsylil derivatives which results in a mass spectrum with very limited information. I don't know about boronic derivatives, they are not very popular, though, so they must have their own drawbacks.