Posted: Sun Jun 22, 2008 6:33 am
by jzt
I thought the trend is exactly the opporsite. more new systems are based on binary/high pressure mixing. the trend is going to "fast LC" with higher pressures and using smaller particles and shorter columns. in my experience the low pressure mixing system always resulted in more baseline noise in such applications, and the dwell volume is to high relative to the column volume. last year our department purchased 10 new units of Agilent 1200 system, all exclusively binary pumps.
Posted: Sun Jun 22, 2008 9:25 am
by zlb215
in my experience the low pressure mixing system always resulted in more baseline noise in such applications.
Why?Obviously,a greater dwell volume is not the cause.Will GPV of solvent be source of baseline noise?
Posted: Sun Jun 22, 2008 2:37 pm
by zlb215
About the trend,I insist my idea.Although many advanced systems(waters uplc,etc.) still use binary pump system,and just like jzt said,low-pressure system have many disadvantages.However,I believe these disadvantage will be eliminated by development of GPV(for baseline noise),in-line degasser(for bubble formation),microsoft(some special treatmen for dwell volume when run a gradient method).
On the other hand,binary pump system have some unavoidable disadvantages :double pump means more expensive and more maintenance work(and money).
Best Regards