Chromatography Forum

Posted: **Mon May 26, 2008 2:48 am**

Can you give us the column dimensions, flow rate, and the details how you bring the solvent to temperature?

Column dimension: 30 x 2.00 mm, with 2.5 um particles.
Flow rate: 1.0 mL/minute.
Column oven: forced air-circulation-type (without pre-heating)

Posted: **Tue May 27, 2008 2:57 am**

My suggestion is to try preheating. It may sharpen up your peak, since temperature is an important factor in the speed of this equilibrium.

Posted: **Wed May 28, 2008 12:24 am**

Thanks, Dr. Neue. Unfortunately, we do not have column oven with pre-heating system right now.

Weird Tailing?