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Posted: Fri Oct 01, 2004 12:39 pm
by Yury Zelechonok
How long is your column?
Is your sample in 85% MeOH as well?
Do you have any other chiral centers in you molecule beside one that shown?
Posted: Fri Oct 01, 2004 1:24 pm
by HW Mueller
What is your dead time?
Also, unless you have some diastereomeric interactions you would not split enantiomers. For diastereomers the molecule seems a bit small (I don´t discern what it is as yet). Also, I would think that it´s rather unusual to have diastereomers elute together to that conditional extend.
Posted: Fri Oct 01, 2004 2:21 pm
by Basil
How you prepare your sample solution ?. What pH ?
Do you use the mobil phase to solve your compound ??..
Try to use a solvent weakness than your initial conditions in your mobil phase