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retention time stability

http://www.chromforum.org/viewtopic.php?t=6892

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Posted: Sat Oct 06, 2007 11:57 am
by zokitano
Rick,

Does your colleague use helium to sparge the m.f. solvents while the HPLC instrument is operating?

best wishes

Posted: Sun Oct 07, 2007 7:02 am
by rick1112
hi
no he doesnt....
its a smiple RP method...using 100% ACn +0.1% TFA and 100% water+0.1% TFA(gradient run)..C18 column...
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