Re: HPLC assay for Neomycin sulfate
Posted: Mon Jun 28, 2021 4:48 pm
What sample concentration, wavelength and flow rate did you use?
Years ago, I developed a method on Hypersil Gold column with TFA+solvent gradient for Neomycin and Gentamicin. I seem to remember that it took a 'much' stronger TFA concentration to separate Neomycin B and C, and Sisomicin from the Gentamicins.