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- Posts: 310
- Joined: Thu Jun 18, 2009 7:59 pm
We have never been able to routinely inject 20 uL using 0.3 mL/min of flow on the 1100 - it just takes too long to all get to the column and ends up causing band spreading. When I run narrow bore (2.1 mm) columns I always drop my injection volume down to 5 or 10 uL. Also, if you run narrow bore columns you need to make sure you have a small volume UV cell - the standard cell will cause you grief with low flows.
Laboratory Director
Krause Analytical
Austin, TX USA