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Downward peaks

http://www.chromforum.org/viewtopic.php?t=3115

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Posted: Fri Dec 23, 2005 10:16 am
by HW Mueller
Peeking in from vacation:
Srinivas, what´s a UV quencher?

Posted: Fri Dec 23, 2005 10:30 am
by Srinivas SK
UV-quenching is used widely in TLC, to detect compounds that do not fluoresce under UV. Done with a fluorescent TLC plate containing small amounts of fluorescein. Under UV , a UV quenching compound would show up as a dark band against a fluorescent green background. Such a compound may show up as a negative peak in an HPLC analysis with UV detection.
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