Posted: Mon Feb 06, 2006 7:36 am
I have tried using more than 40 column volume this morning. The result is almost the same; Tailing factor (10%) = 1.49 (by the software). However, I think it is better than before.
The condition is different. Here is the mobile phase:Syx, I did not say without buffer. Actually, you may be better off with the buffer. Are you using the exact same conditions as posted above by Peps?
Also for hwo long do you need to store the column? If you plan to staore the column for half a year, put it in acetonitrile. If you want to reuse the column after two days, keep it in the mobile phase.
It is about column storage as we talked about. The frequency of reuse is depended on the production schedules.Mobile phase – Dissolve 2.0 g of sodium 1-hexanesulfonate in 800 mL of pH 6.8 buffer, adjust with 0.5N sodium hydroxide or 0.5M phosphoric acid to a pH of 6.8 +/- 0.1, and dilute with pH 6.8 buffer to make 1000 mL of solution.
