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Inconsistent rentention time HELP. URGENT~!!!

http://www.chromforum.org/viewtopic.php?t=26343

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Re: Inconsistent rentention time HELP. URGENT~!!!

Posted: Fri Mar 20, 2015 5:52 am
by richiekichi
If you inject the same standard repeatedly do you still see the retention time drift?

Your differences in RT seem pretty negligible to me. Especially over such a large concentration range. But then I don't work in a regulated environment or with nice clean samples.

Re: Inconsistent rentention time HELP. URGENT~!!!

Posted: Fri Mar 20, 2015 6:40 am
by paulinephoon
If you inject the same standard repeatedly do you still see the retention time drift?

Your differences in RT seem pretty negligible to me. Especially over such a large concentration range. But then I don't work in a regulated environment or with nice clean samples.
Thanks for your opinion, richiekichi.

I lower my concentration and its range, so the difference RT wonldn't be so large.

Problem solved :)

Re: Inconsistent rentention time HELP. URGENT~!!!

Posted: Tue Mar 31, 2015 9:43 pm
by JMB
Apart from the issues already mentioned, decreasing retention time with increasing concentration may be due to column overload.
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