Old Thermo HPLC

[uzman]

Actually , I don't know the name of the software that is compatible with OS/2 , since it is a really old one.
I am using Chromquest 4.1 under Windows XP.

I suggest you may obtain the necessary chip from Thermo-Fisher Scientific.

[emmet]

Dear Uzman,

I agree completely with you, and as soon as I find a chip I letting you know if it works.

Exceedingly thank you.

[emmet]

I finally got the famous chip, and when I use the software I was able to achieve communication only with the pump and autosampler, but not with the detector. Detector that I have is UV 1000. On the other hand, the Xcalibur software version that I have offered only UV 2000 to be installed, so I wonder if this is the main cause why the program does not recognize the detector or is it something else?

Thanks in advance.

[uzman]

Hi emmet,

It is good to hear that you succeeded !
I don't know the details of Xcalibur software so I have no idea about the problem.

The difference between UV-1000 and UV-2000 is the wavelenght range for their deuterium lamp.
UV-1000 has a range of 190nm to 380nm and UV-2000 has a range of 190nm to 365nm.
W lamp is optional for UV-1000 and standard for UV-2000.

[emmet]

Hello Uzman,

I'm very glad you again helping me, and also very grateful for that. Today I tried again to run my HPLC system, and I failed. However, I guess I realized what was wrong. The cable from the controller to the detector seems to be rolled and not a classic like the one for the web. Since the LAN cable from the computer to the controller SN4000 is rolled, I tried to connect with this cable detector and a controller, and than the detector restarted by checking with its components. Unfortunately I did not have another cable of the same type that would connect the computer and the controller. So I hope that when I create another cable of the same type, all will start operating. By the way, can you tell me what kind is your cable that connects the controller and detector?

Thank you in advance.

[uzman]

It is 8 pin cable , same as which connects the controller to the pump and the controller to the autosampler.

[emmet]

Dear Uzman,

Would you be so kind to tell me the order of wires on both ends of the cable which connects the detector and controller. It would be nice if you would draw a schematic in the same way as for the adapter. This is my last idea why the controller and detector are not communicating.

Thank you in advance.

[emmet]

I finally understood that the cables are not the problem, but drivers for UV 1000 detector are missing. The current version of the software that I have only supports UV 2000 detector. Would someone be so kind to upload drivers for UV 1000 detector (firmware 3.09).

Thanks in advance.

[wally]

Hello emmet,

I have similar hardware as yours and want to start using it.
Two questions in front:

On top of this thread you asked for:"RS232 to DB9 adapter"
My RS232 is a DB9, on PC and on SN4000
and later in uznan reply: "9 pin RS232 8 pin DB9"
What is a "8pin DB9"

All other cables from the SN4000 are RJ12 6P6C
http://www.suntekpc.com/htm-2/connector ... 48-mmj.htm

I assume the communication between SN4000 and TSP UVxxxx are serial protocols similar or identical to RS232.
Can you agree with this ?
and do you know the pinouts ?

Later in thread thee is a link to a adaptor wiring,
http://i62.tinypic.com/34y8tch.jpg
but this is RJ45 8C to DB9. I have only 6 wires between SN4000 and e.g. Detector.

Did you try Datasystems other than XCalibur ?
Does your system work now ?

cheers wally

[uzman]

Hi Wally,

8 pin DB9 is a wrong statement , sorry , it is actually a 8 pin RJ45 socket.

[wally]

Hello uzman,

thank you for reply and making things more clear. Anyway I have this smaller 6pin (RJ45) but I think I can figure out which are the important wires.
Probably the inner 2 for Rx and Tx and some wire for ground (?)

Can you say anything about the protocol between the PC <-> SN4000
and the communication between SN4000 and detector ?
I fear I must try to drive the instruments from linux with some own software.

Do you actually work with your Thermo instruments ?

[wally]

another consideration:

The 6 pin (rj45) connectors from SN4000 fits perfectly to
UV6000 and UV1000 and it looks for me as an 8 pin connector
would not fit.
Are there different connector types on UV6000 and/or UV1000 ?
May you be so kind and confirm, you really have 8 pin connectors
on your SN4000 and on detector side , please.

thank you

[uzman]

Hello Wally ,

If you look inside the SN4000 , you will see there RJ45 sockets inside.
3 of them 6 pin RJ45 , connected to instruments , one of them 8 pin RJ45 connected to computer.

[wally]

Good morning Uznan,

yes, much more clear now , thank you.
Do you know any details on the serial communication protocol
between SN4000 and PC ?

Are there details known about communication between SN4000
and instruments ?

[uzman]

Sorry , I don't know the communication protocol , but since they are communicating using RS232 , you may use the wiring diagram ( RJ45 to DB9 ) I uploaded.

You can get the RS232 connections from Google for DB9.