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Re: Impurity in HPLC Water
Posted: Sat May 05, 2012 10:33 pm
by carls
If you leave the vial number blank (no entry) the Agilent 1100 will perform a no injection run. The valve will NOT move from inject to load to inject as in a normal run which eliminates the injector rotor/stator as the source of the problem. The loop is always inline unless you run an injector program.
Re: Impurity in HPLC Water
Posted: Tue May 08, 2012 9:32 am
by thekartis
Re: Impurity in HPLC Water
Posted: Tue May 08, 2012 11:06 am
by unmgvar
we had a weird case of a negative peak in the chromatogram kind of like that.
it was stupid really, and we have no idea why it was the behavior we had,
but someone made the runs on a waters RI while the purge was "on" and it gave a negative peak somewhere
Re: Impurity in HPLC Water
Posted: Tue May 08, 2012 11:53 am
by Uros
I would say, that you have contamination in sampler area (Loop, metering device, needle, needle seat or valve). Try heating water to around 70 Degree celsius, bypass the degasser, and flush the system through the valve position in main pass. Use zero-dead volume insted of column and set the thermostat to max.