Re: Ammonium acetate buffer pH=4 problems
Posted: Fri Mar 23, 2012 1:33 pm
It seems like the pH is my main problem. In the lab we have 3 pH-meters and sometimes, though they are calibrated, they show different pH within 0,1-0,3 pH units for one same solution. When my ammonium acetate buffer is set on a lower pH value 3.85 for example, that's when I start to deal with increased retention and problems begin...
Thank you everybody for sharing your thoughts.
Thank you everybody for sharing your thoughts.