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Re: Conditioning sorbant in RP-SPE
Posted: Wed Jan 18, 2012 12:47 am
by tom jupille
What is the origin of these so-called "active sites"? Are these hydrolyzed silanols?
Arguably yes.
In regard to answers to your original question, though, there's something of a "blind men and the elephant" syndrome going on here. That initial conditioning probably does a number of things, and each of us focuses on one aspect!
Re: Conditioning sorbant in RP-SPE
Posted: Wed Jan 18, 2012 12:41 pm
by DJ
I'm still curious to understand more about the conditioning step, particularly when the reasons for conditioning a C18 SPE don't seem to apply to PSDVB, yet, PSDVD SPE sorbant needs conditioned. Perhaps this step allows modest swelling, better access to pores?
Re: Conditioning sorbant in RP-SPE
Posted: Sat Jan 21, 2012 8:12 pm
by Don Shelly
You are correct.
Re: Conditioning sorbant in RP-SPE
Posted: Thu Aug 09, 2012 4:55 am
by Edde
Is there any difference if I apply 2 changes of MeOH instead of 1 change and also 2 changes of H2O versus 1 in the subsequent conditioning step for C18 SPE?