Re: oligonucleotide -protein conjugate
Posted: Fri Oct 28, 2011 2:04 pm
by Ting Wu
Hi Zahra, SAX would work. SEC might work depends on the size difference. Would you please suggest the size of the tag and DNA, besides the 30 KDa protein?
Ting
Thanks Ting. I am looking at SAX columns for their characteristics. The DNA size with tag is between 4-5 nm and protein is a monomer with 3X4 nm size.
Zahra
Hi Zahra, I believe both SAX or WAX would work. You can try SEC as well. We also offer column screening service if needed:
http://www.sepax-tech.com/services_method.php. Good luck!
Ting
Re: oligonucleotide -protein conjugate
Posted: Thu Mar 31, 2016 12:28 pm
by annm
hi guys!
i am facing the very similar problem right now: I need to separate protein (28kda) from oligo-protein (38kDa) and oligo-protein-oligo (48kDa). Ideally i want 3 peaks to be present on the chromatogram.
ordinary size-exclusion with FPLC didn't work for me (Superdex 75 10/300 GL) - i got one smeared peak.
Zahra, have you solved your problem or have you found a way around it?
does anyone else have a hint what method to try?
Re: oligonucleotide -protein conjugate
Posted: Thu Mar 31, 2016 2:21 pm
by Andy Alpert
Anion-exchange, same as my response to Zahra.