oligonucleotide -protein conjugate

[Ting Wu]

Hi Zahra, SAX would work. SEC might work depends on the size difference. Would you please suggest the size of the tag and DNA, besides the 30 KDa protein?

Ting

Thanks Ting. I am looking at SAX columns for their characteristics. The DNA size with tag is between 4-5 nm and protein is a monomer with 3X4 nm size.

Zahra

Hi Zahra, I believe both SAX or WAX would work. You can try SEC as well. We also offer column screening service if needed: http://www.sepax-tech.com/services_method.php. Good luck!

Ting

[annm]

hi guys!
i am facing the very similar problem right now: I need to separate protein (28kda) from oligo-protein (38kDa) and oligo-protein-oligo (48kDa). Ideally i want 3 peaks to be present on the chromatogram.

ordinary size-exclusion with FPLC didn't work for me (Superdex 75 10/300 GL) - i got one smeared peak.

Zahra, have you solved your problem or have you found a way around it?
does anyone else have a hint what method to try?

[Andy Alpert]

Anion-exchange, same as my response to Zahra.