Re: Expiration of aqueous mobile phase?
Posted: Mon Apr 25, 2011 1:57 pm
I think this approach can have a drawback: If you start with 95%A and 5%B in the morning, at the end of the day, there may be some 4% of B because of evaporation.This is another good reason to pre-mix solvents. If your gradient method starts at 95%A and 5%B, rather than using a gradient pump to achieve this just pre-blend your A solvent to that condition and adjust your gradient settings or B solvent to compensate.
This should help stave off bacterial growth and also gives you lower noise in your chromatography.