Chromatography Forum

Ah ah ah...I have 3 LCMSMS from Thermo, so you can imagine my suggestion...)
Consider that Tracefinder is now very far from Waters software.

My suggestion is to organize a demo with both with a very dirty real sample, like I did in the past. In this case Thermo was better because of its heated ESI and the possibility to work in H-SRM mode.
The library of thermo are quite complete (for what I have to do), and Tracefinder is fast enough to reprocess a lot of sample every day.

H-SRM mode is really so good?
If i'm right, in HSRM we can reach 0.4 amu resolution, waters TQD is very near to 0.4 (I don't remember the exact value, but I remember that Waters TQD can reach similar performance...).
Last question: do you really think that tracefinder is very far from waters software?

I was mistaken, I wanted to say that Tracefinder is not very far from Water software..
H-SRM mode, made with hyperbolic quadrupoles is really good. If Waters does it, it will loose 70% of Ion transmission and sensitivity.
Try it on real sample so you'll see the difference.

Thank you.
What kind of analysis do you do with you thermo lc/ms/ms?
Do you have Accela and Quantum Access MAX?
Last question: do you have experience on APPI source?
Thanks
Wesley

I have two TSQ Vantage and one Access Max, all with Accela 1250. I use them mainly for food safety analysis: pesticides, toxins, veterinary drugs, etc...

Thank you.

It is a very important decision

Please give me your opinion...

And what's about agilent quaternary pump?

We have both the Thermo and Waters in our lab. Reliability wise, both have been very comparable. The Waters software works better for our use, but every lab is different. Personally I lean a little more toward the Waters, but it is just personal preference.

We have Waters TQD, LCT Premier XE and Quattro Premier, AB 3200, and Thermo TSQ Vantages. The Thermos are generally more problematic for us and not so good on sensitivity. The AB mass specs are very reliable, but sometimes do not fragment as well as the Waters due to the difference in collision gases and also the collision cell in the AB 320 is nowhere near as good as the TWIG collision cell in the Waters. Acquity gives good performance but is very problematic - constant breakdowns and maintenance. Shimadzu has a new UPLC system, and they tend to make very reliable pumps. The Agilent triple quads are not very good in my experience. To be honest, the AB 5500 or Waters TQS are both very nice systems - sensitive, reliable, stable. Also, I'm not sure why everyone on here keeps saying Waters does not have a heated ESI - there is a heater in the ESI probe and heats the desolvation gas and eluent, and there is a heater in the ion block of the source. They do not heat as high as the AB Turbo V source, but it is heated and does pretty well especially with thermally unstable compounds. MassLynx is easy to use, but so is Analyst. I haven't really used Excalibur much. I would definitely check with people in your area as far as their satisfaction with service, because any of these is going to break down someday. I personally would check out the new Shimadzu and either the AB 5500 or TQS and try to haggle the price.

Hello, I need your help: i'm looking for a lc/ms/ms system, triple quad.
I valued many purposes, and now i'm deciding which of these two is better (I must decide tomorrow)...
-waters uplc aquity (binary pump)/ tqd mass spectrometer, esi/apci combined source;
-thermo uhplc accela 1250 (quaternary pump)/ quantum access, esi/apci combined source.
I will use that for drugs of abuse discovery, pharmaceutical, multiresidual, aflatoxins, ocra, ecc...
I need an easy to use system, user-friendly, with good performance.
What's the better? The price is exactly the same.
Thanks.

Thanks god you have choices.
99.99% of us do not have choices, we have to live with equipment that did not work well for the previous person and we are stuck with equipment without service contract and support, and the expectation from us is to do mircles.
Most employers do not want to spend any mony on good support.
The employers understand that the systems will solve any issue.
Get the system which is the most dificult to work with. Your job would last longer because you would not get the answers. If you do get the answare, 99.99% chance that your employer would not like the scientific fact, and eliminate you assuming that the aflatoxin you find out in their product would be fired with you.

Hi, everyone.
I have read this topic because I'm about to get a LC/MS/MS and I still don't know which one to choose.
I don't have so much money avaiable. I quoted LC/MS/MS from Applied BioSystem (they use Agilent UPLC), but it's too expensive and I can't afford it. I have talked to Bruker and they offered me a Shimatzu Nextera UPLC and Bruker Q-TOF. it's expensive, but cheaper than applied. Do you beleive LC q-TOF is useful for quantitation? I work with drugs metabolites in serum and urine, pestcides in food and I need very good sensitivity.

Other options I have thought of are Thermo Quantum Acess and Agilent 6430 TQD. Do you have an idea how much the whole system (UPLC included) cost (Thermo and Agilent)?

I appreciate if you can help me.

The price of TSQ Access Max and Agilent 6430 is quite similar...about 130k euros with UPLC

Thanks for replying me.
What about UPLC + Applied MSMS (q-trap). Do you know the avarage price?