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Posted: Wed Feb 02, 2005 8:17 am
by HW Mueller
It seems there got to be a way to get rid of the TFA, even if you have to use some more solvent. If you use a solvent with less elution power than mobile phase you can inject much more volume. Uwe worked out something like that?
Posted: Wed Feb 02, 2005 8:20 am
by HW Mueller
It seems there got to be a way to get rid of the TFA, even if you have to use some more solvent. If you use a solvent with less elution power than mobile phase you can inject much more volume. Uwe worked out something like that?
Posted: Wed Feb 02, 2005 1:51 pm
by Alex
Great! I doubled the buffercontent from 0.5 % AcOH to 1 % AcOH and the peakshape is much better.
Additionally I swiched to the Nucleodur RP-C18 CN column (pyramid) wich helped also.
Now I will try to raise the flow in order to "squeeze" the peak together.
This guides me to another question: Is there a reasonable border to which raising the buffercontent doesnt make sense anymore? Because I never red of buffervalues over 1 %.
Thank all of you for your help!!!!!!!
Posted: Thu Feb 03, 2005 8:39 am
by kiknos
Great! I doubled the buffercontent from 0.5 % AcOH to 1 % AcOH and the peakshape is much better.
Additionally I swiched to the Nucleodur RP-C18 CN column (pyramid) wich helped also.
excuse me, what is RP-C18 CN column? mixed C18 and CN as stationary phase? i am curious.
Posted: Thu Feb 03, 2005 9:33 am
by Alex
It is a hydrophilic endcapping which enables a mobile phase with 100 % Water without collapsing of the C-18 chains. See also here:
https://www.macherey-nagel.de/web/MN-WE ... NDURC18PYR
Regards,
Alex
Posted: Thu Feb 03, 2005 11:31 am
by Steve
There is a great deal on the Pyramid and Gravity Columns offered By Macherey-Nagel. If you check the Announcement Board of this forum Macherey-Nagel is offering a Buy One get One Column free until 02/15/2005.
Posted: Fri Feb 04, 2005 3:44 am
by kiknos
Alex and Steve, thanks for the kind information!
one problem for me is everytime i tried to load M-N web site, i always got safety warrenty. so i had to give up as i know so little about computer and net and i don't want to get any virus or safety risk.
Posted: Fri Feb 04, 2005 2:32 pm
by MG
I get that warning too. Something about the name on the Verisign certificate not matching the site name. I think this would only be a security issue if you were going to order stuff through the site using a credit card number etc.