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Posted: Wed Jun 09, 2010 6:38 pm
by yaakov
Uve Neue,
you are right, UV 190 is to low for 50:50. PH =5.5
Unmqvar
I do not understand the abbreviations MB and BTW.

Posted: Wed Jun 09, 2010 7:25 pm
by unmgvar
MB is a typo mistake for MP= mobile phase
BTW is "by the way"

Posted: Thu Jun 10, 2010 1:55 am
by Uwe Neue
If you want to see your analytes in UV, you either need to use a phosphate buffer or no buffer. Sorry for insisting that HILIC on silica might be the better solution.

Posted: Thu Jun 10, 2010 3:30 pm
by yaakov
Uwe Neue,

your advice is greatly appreciated. I`ll try and report.

yaakov