Re: anyone can recommend any review on LH-20
Posted: Tue Aug 23, 2011 2:38 am
by goldita
it is odd that people do not recommend use of mixture solvent,only use one pure solvent. how can they gurantee complete elute of analytes in sample?
do you guys think LH-20 stationary phase is similar to cyno column?
You can *never* guarantee complete elution in liquid chromatography.
Can anybody have idea about removing attached contaminant from LH-20 column bed because after running samples in LH-20 column, I got my LH-20 coulmn colorized. So I want to remove the color. If anybody has idea about this then please replya s soon as possible.
Thank you so much
Re: anyone can recommend any review on LH-20
Posted: Mon Jan 19, 2015 10:02 am
by carlo.annaratone
This is a very old topic, but I recently started using Sephadex LH-20 therefore I encountered the same problems mentioned in the thread. So far I found exceptionally useful the following book:
Preparative gel chromatography on Sephadex LH-20
Hans Henke
Huthig Verlag, Heidelberg, 1195
ISBN: 3-7785-2428-3
It is an eminently applicative book, therefore one will not find lengthy discussions about the underlying theory of separation modes on LH-20. I think this is because the behaviour of the material is so complex (gel filtration + lipophilic + hydrophilic + partition...) that simply there are not enough studies around to accurately make a theory out of it. However it gives practical rules about setups, solvents, sample types, and elution order of real world samples that will make life definitely easier