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Posted: Sun Jan 17, 2010 5:16 pm
by danko
if this was the case then it should continue to occur, right..?
Depends on how you test it.

Aside from that; did I get the the eluents and the gradient protocol right?

Best Regards

Posted: Sun Jan 17, 2010 9:28 pm
by DJ
What is the theory behind the NaOH column regeneration? Can peptide bonds really be hydrolyzed at ambient temperature?