Chromatography Forum

Dear HW Mueller,

It looks that you are trying to help me in the best manner. Thanks for this.

I would like to ask you what is your vision for further alteration of chromatographic conditions.

In addition, a selected wavelength of 275 nm corresponds with literature data and the published spectras of naphthalene. If you have some other ideas about the Excitation wavelength, please share them with me. The combination (Excitation/Emission) was chosen due to the higher peak signal of naphthalene. However, the signal does not break world records for size.

I can not remember a method for a sample enrichment of water:acetonitrile solution containing naphthalene (or whatever is there)without using SPE for instance. I belief that using of an additional method for enrichment should not submit new doubt on the obtained results.

Thanks in advance for your valuable comments and suggestions.

First of all, I forgot to ask this before: Isn´t it possible to collect some peaks and submit them for MS somewhere?

How about setting up a NP with a silica column, or GC, collecting peaks and giving them away for an MS.

Good to hear that you can select other wavelengths than 275. If your apparatus does not allow scanning the wavelengths at least you can use a bunch of different excitation wavelength and observe whether the emission changes the same as when a naphthalene standard is in there.

It could solve your problem if you answered the question on whether you were ever able to restore the original rt.

If your peak is naphthalene it means you are contaminating everything around. If you can not stop this an SPE wouldnt help.

It's an old method, but maybe it will help DID in eliminating his possible napthalene contamination...

http://www.caslab.com/EPA-Methods/PDF/8310.pdf