Posted: Wed Oct 07, 2009 5:16 pm
Bigbear:
I am certain it is a cold spot, as I can eliminate the taling completely when I heat the tubing in question.
The GC conditions were developed by someone else, and I am attempting to preserve as much of the original settings as possible (this is a method transfer project in a GMP environment.) I agree that the conditions are not optimum (to be kind), but changing them would not be allowed. As it is, I am changing some operating parameters to account for the instrument differences, and that is about all I can justify.
I am certain it is a cold spot, as I can eliminate the taling completely when I heat the tubing in question.
The GC conditions were developed by someone else, and I am attempting to preserve as much of the original settings as possible (this is a method transfer project in a GMP environment.) I agree that the conditions are not optimum (to be kind), but changing them would not be allowed. As it is, I am changing some operating parameters to account for the instrument differences, and that is about all I can justify.