"Dilute and Shoot"

[kerri]

CPP is one, the others are analogs. Chemists are such good detectives!

Sounds as if you have worked with the compound before. Any advice?

Cheers!

[Stryder08]

CPP is one, the others are analogs. Chemists are such good detectives!

Sounds as if you have worked with the compound before. Any advice?

Cheers!

I routinely screen for BZP, mCPP, TFMPP, and about 400 other compounds in postmortem blood and tissue specimens. We utilize UPLC/time of flight mass spectrometry and use a quick gradient of 0.05% Formic Acid in DI Water and Methanol as mobile phases. The column is a Waters Acquity HSS T3, 2.1 x 100 mm, 1.7 um.

My advice is to dilute your specimen in something that is similar to your mobile phases. We crash the specimen with ACN and then dilute 50:50 with DI Water.

I take it you are using HPLC-MS?

Fun fact: mCPP is the primary metabolite of two common antidepressants, Trazodone and Nefazodone.

[kerri]

Using LC-ESI-MS/MS (but developing the detection/separation method on a different instrument here via SIM).

I am happy to hear you crash with ACN and then dilute. That is exactly what I am doing while I par-develop the method by drying and reconstitution, as well as direct ACN super injection. Just covering all of my bases ( I know this seems quite ridiculous (time-consuming), but I'm taking this as a learning experience. I have only developed one other method.. trying to pack some experiences into my brain before I graduate!)

faciem at gmail

[Uwe Neue]

Since you are new at this, some advice:
Watch out for ion-suppression! Coeluting residual protein can cause ion suppression (or enhancement) and fake the results. If you run into this problem, you need to improve the sample prep method to get rid of interferences. If the problem does not occur, move on...

[kerri]

Thank you for the advice Uwe!

[Baxthorpe]

Horses for courses.
Remember, Kerri, there's differences in precision with the detection methods.
Using time of flight, you only need a slight separation to be able to resolve things in the detector ...

[kerri]

Thank you so very much Baxthorpe!!