-
- Posts: 2846
- Joined: Mon Aug 30, 2004 7:17 am
Advertisement
Cyclic UV detector noise - not a pump problem
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
20 posts
Page 2 of 2
CPG, you had stop and go oscillations due to the lamp, yet there was enough light output during an oscillation phase to produce such large peaks as Conny sees them?
-
- Posts: 3594
- Joined: Mon Aug 30, 2004 11:43 pm
HB - in my case when I saw the oscillations in the baseline upon initial equilibration before starting the sequence, I didn't go further and inject any samples/standards. I fixed the problem.CPG, you had stop and go oscillations due to the lamp, yet there was enough light output during an oscillation phase to produce such large peaks as Conny sees them?
I have also seen small oscillations which, when compared to the size of the sought-for peak, were negligible.
-
- Posts: 2846
- Joined: Mon Aug 30, 2004 7:17 am
What I would like to know is, whether anybody has seen oscillations due to a dying lamp (not due to electrical interference, etc.) which still had enough light output to create huge absorbances (peaks). Also what would cause a dying lamp to oscillate in repetition?
Conny, don´t you have a new lamp? (The trouble is, by the time the new lamp is put in the air problem could have resolved itself).
Conny, don´t you have a new lamp? (The trouble is, by the time the new lamp is put in the air problem could have resolved itself).
-
- Posts: 762
- Joined: Thu Dec 13, 2007 5:54 am
Yes, I have had a lamp go intermittently bad on me while still producing enough power to generate peaks of interest. While the only chromatogram we've seen is during a run, we can consider that the chromatographer has run a 'flow-off' situation with similar events. The easiest solution is flushing the flow cell and re-evaluating the error, although I consider a bubble in the flow cell a rather unlikely occurence. Based on what I've seen, a lamp power fluctuation is the cause, and it is very easy to swap a lamp and re-evaluate a problem. If the problem "goes away" after swapping the lamp, it is a throw away excuse that the "air bubble" went away following the lamp replacement.
-
- Posts: 2846
- Joined: Mon Aug 30, 2004 7:17 am
bisnettrj2, ok, I see that this has to be approached differently if I am not to go away from this discussion with another myth engrained in my head:
What was the S/N ratio of your "peaks of interest" during a bad phase of the lamp?
I still don´t know about the oscillations.
What was the S/N ratio of your "peaks of interest" during a bad phase of the lamp?
I still don´t know about the oscillations.
20 posts
Page 2 of 2
Who is online
In total there are 175 users online :: 1 registered, 0 hidden and 174 guests (based on users active over the past 5 minutes)
Most users ever online was 5108 on Wed Nov 05, 2025 8:51 pm
Users browsing this forum: Google [Bot] and 174 guests
Most users ever online was 5108 on Wed Nov 05, 2025 8:51 pm
Users browsing this forum: Google [Bot] and 174 guests
Latest Blog Posts from Separation Science
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.
- Follow us on Twitter: @Sep_Science
- Follow us on Linkedin: Separation Science
