Chromatography Forum

We actually do an extraction on the sample with water.

SiliCylce/Charles
I've had no problems with C18 columns, and I've developed two aqueos low UV methods. I have proposed the idea of buying some different columns to my manager, and she agrees, but we are undert a tight budget this year. If we did a lot more LC than we do we might be able to make a better arguement. We actually have only one HPLC, and we are supposed to be getting a second from one of our other facilities. If we are going to be running two at a time, I can probably make more of a justification for getting some additional columns. I would definately consider going to a better column for 100% aqueous.

Hello Tim,

Let me know for the futur regarding low prices for HPLC columns. I know good HPLC column for most popular applications: for low and high pH, 100% aqueous etc. Those columns are equivalent or better than Waters and Agilent, but cheaper.

Good Luck
Charles

Well, we have a case here where a person who does not know what he is talking about is trying to badmouth a group of well established poducts. Not a good sign.

The Atlantis family of products from Waters, Atlantis dC18 and Atlantis T3, has been specifically developed for use in mobile phases with 100% water, i.e. they do not require the addition of an organic solvent to the mobile phase. Any claim to the contrary is simply nonsense and shows that Charles SiliCycle needs to familiarize himself with the products that are on the market.

I am sorry I worked for Waters for more than 10 years so I know perfectly the Atlantis chemisty. And after I worked for couple CRO using Atlantis and We had many problems for reproductibility with higher aqueous in mobile phases. It just my experience, also lot of ion supression with LC-MS triple quad as API 4000 and API 5000.

Again, I know Waters they make other types of Atlantis in order to improve thoses issues, but it is very expensive.

Sorry

The reason that we have developed a second-generation product, the Atlantis T3, has absolutely nothing to do with your claim that it would not work in 100% water, which is where this discussion started. It worked very well, but improvements are always possible, especially if one has a strong R&D department that is capable of making significant product upgrades.

I did my own research and contacted Waters directly about it, and the T3 Atlantis which is what I have should be good for a long time, provided it's taken care of (it is) and the quality of the samples we run through them.