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- Posts: 374
- Joined: Fri Oct 22, 2004 7:14 pm
This may sound silly, but make sure you have the right concentrations for the internal standard and 1,1,2,2-tca in the calibration table and you are using the correct ions for quantitation.
I doubt split ratio will help the situation because the split ratio won't change the mass ratio of internal standard and target compound injecting into the column.
