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Very good. Just test as my suggested. The resoution should improve, even significant，and retention would be longer.My two compounds are allopurinol and oxypurinol with pKa values 10.2 and 7.7 respectively
Thankyou for your suggestion, i will try....parveenqa,
as stated earlier,
see your capacity factor situation.
your compounds have nealy zero retention on your c-18 column and this because they are very polar, because of this you have very small chances of getting a good separation between them, also for robustness and accuracy conditions it is best to be with a greater capacity value. above 2 is preferable at least 1 is good. a good chromatography method will take this into account, from there separation will be easier to achieve
now that we know what they are exactely, i would suggest either trying a polar embeded c-18 or you should move to a HILIC or normal phase condition; preferably HILIC in my view.
No, these two are not tautomeric forms of each other. oxypurinol is the metabolic product of allopurinol, formed after oxidation...Very good. Just test as my suggested. The resoution should improve, even significant，and retention would be longer.My two compounds are allopurinol and oxypurinol with pKa values 10.2 and 7.7 respectively
By the way, they are -one and di-one, why call -ol? tautomerism?
No, these two are not tautomeric forms of each other. oxypurinol is the metabolic product of allopurinol, formed after oxidation...
Thankyou
Oh sorry, i got it...No, these two are not tautomeric forms of each other. oxypurinol is the metabolic product of allopurinol, formed after oxidation...
Thankyou
No, No, I didn't mean they are tautomerism each other. I mean each of them has its own related tautomeric -ol because they are really-ones(please see their structures), but call -ols. But just foget it.
Thankyou for your information, i think that will be very helpful....I guess this application might help you.
http://www.waters.com/webassets/cms/lib ... 00.308.pdf
I have analyzed allopurinol and xanthine before using LC-MS/MS. I used Waters Atlantis T3, and I was able to separate 2 compounds.
Best Regards,