method validation for different matrixes

[MK]

Hello,

I'm quantifying one active from three different matrix compositions as follows:

Matrix 1: Contains components A and B and the active
Matrix 2: Contains components A, B and C and the active
Matrix 3: Contains components A, B, C and D and the active

am I right saying that I can perform specificity, linearity, accuracy etc. only on matrix 3 and the method will be valid also for matrix 1 and 2?

Another thing; I'll be using 100 ul injection routinely. However rarely I may need to use larger injection volume e.g. for diluted samples. Which validation tests should I repeat for the high volume extension?

thanks

[pawan ratra]

Precision should be carried out for each formulation.
Linearity and recovery can be combined.
Specificty should be handled very carefully. Degradation studies should be performed for individual formulation.
Solution stability-For each formulation.
Robustness can be performed in formulation containing all components.