Silica column - please help

[Andrea Hilton]

Dear members,

Can anyone suggest me a way (if it does exist) of restoring a silica column. Someone has washed it by mistake with water (for a long time I'd rather don't know how long is it) and I don't know if it will survive. I need the column to run an analisys in 2 days and I'm worried because it is the only silica column I have in my laboratory.

Regards,

Dea

[tom jupille]

Switch to pure methanol and then work your way back down the polarity series until you get to your desired mobile phase.

[okara]

you can use ACn or methanol and run for 2 to 3 hours then your come back to same condition but in future if you use high % of water then use Floro column
thanks

Dear members,

Can anyone suggest me a way (if it does exist) of restoring a silica column. Someone has washed it by mistake with water (for a long time I'd rather don't know how long is it) and I don't know if it will survive. I need the column to run an analisys in 2 days and I'm worried because it is the only silica column I have in my laboratory.

Regards,

Dea