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- Posts: 28
- Joined: Mon Sep 19, 2011 1:16 am
Is it necessary to Use TEA when my buffer pH is 2.3 and my analytes are acidic in nature ?
In my case, the peak shape become good with TEA when compare to without TEA. But I didn't understand Mechanism of TEA in lower pH. Because silanols pKa is around 3.8 (so at pH 3.8, Silanols will be 50% ionised) and as we decrease the pH, ionisation of silanols will decrease. So i didn't see any strong reason to use TEA in my case though its giving me good peak shape
.
While pH is 2.3, is TEA bind with the Silanols
?
What about using Trifluoroacetic acid? there can be any benefical effects in peak shape? I need the mechanism how they are going to act ?
In my case, the peak shape become good with TEA when compare to without TEA. But I didn't understand Mechanism of TEA in lower pH. Because silanols pKa is around 3.8 (so at pH 3.8, Silanols will be 50% ionised) and as we decrease the pH, ionisation of silanols will decrease. So i didn't see any strong reason to use TEA in my case though its giving me good peak shape
. While pH is 2.3, is TEA bind with the Silanols
? What about using Trifluoroacetic acid? there can be any benefical effects in peak shape? I need the mechanism how they are going to act ?
