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Ethylene Glycol in the Sample Matrix

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

2 posts Page 1 of 1
I have around 10% ethylene glycol and 90% water in my sample matrix.
I plan to run LC under the following conditions: Column - C-18, mobile phase - 0.01 M ammonium phosphate at pH 2.8, Injection Volume - 10 microL, UV Detector - 200 nm. I am analyzing Lysine in this sample.
Is 10% ethylene glycol going to clog, or damage my column?
Is 10% ethylene glycol going to clog, or damage my column?
Not likely.
2 posts Page 1 of 1

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