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- Posts: 4
- Joined: Wed Mar 23, 2011 4:30 am
Thanks everybody!
Natalie
You centrifuge the sample and filter the supernate and then test that by headspace.Since we are talking about fermentation broths here, which can vary from consomme to chunky vegetable depending on how well the bugs grow,
I know from experience that works and that hydrolysis is not an issue, at least if the HS oven is in the 60-70C range and you don't have to equilbrate too long (which depends in part on the volume and vial size you use.I am more optimistic than Rod about acetic acid by GC. The lower limits will be higher than for the alcohols, and the repeatability not as good, but much will depend on the concentrations that you need to get down to. You will need to lower the pH with a strong acid, and that might give some issues with hydrolysis of other components.
- Karen
