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immobilized stationary phase

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
Is any one know what is immobilized stationary phase in HPLC chiral column.
what is difference between coated and immobilized stationary phase.
In Chiralpak AD packing composition is
amylose tris (3,5-dimethyl phenyl carbamate) coated on silica .
Where as in Chiralpak-IA it is
amylose tris (3,5-dimethyl phenyl carbamate) immobilized on silica.
what is the diffrence?
"Coated" is just what it sounds like; the layer of stationary phase is like a coat of paint on the support. It is not chemically attached and so is somewhat fragile.

The immobilized phase is chemically bound to the substrate.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
Thank you very much sir.
3 posts Page 1 of 1

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