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Sample solvent for method development

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Sample solvent for method development

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annaand
I have a very basic question: While developing an RP-HPLC method by increasing an aqueous phase percent in mobile phase, what solvent should be used to prepare sample? Richer in organic or aqueous phase?
Anna Andrzejewska-Santiso

Re: Sample solvent for method development

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tom jupille
Site Admin
In general, the diluent should be the same as the mobile phase. When that is not possible or practical, more aqueous is better.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
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