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- Posts: 15
- Joined: Wed Jan 19, 2005 1:22 pm
Dear colleagues,
I try to enrich phosphorylated peptides (or proteins) prior to mass spectrometry. I have so far tried an IMAC HPLC column kindly provided by Dionex and the ProteoExtract Phosphopeptide Capture Kit (also IMAC from CALBIOCHEM). Both these approaches seemed to work, @ least with standard substances, however, only when using rather large concentrations (such as 40pmol absolute entering the respective process).
Any experience in this field and thus any “helpful kicksâ€
I try to enrich phosphorylated peptides (or proteins) prior to mass spectrometry. I have so far tried an IMAC HPLC column kindly provided by Dionex and the ProteoExtract Phosphopeptide Capture Kit (also IMAC from CALBIOCHEM). Both these approaches seemed to work, @ least with standard substances, however, only when using rather large concentrations (such as 40pmol absolute entering the respective process).
Any experience in this field and thus any “helpful kicksâ€
