Best mobile phase to use

[menlite]

Hello,

I'm carrying out HPLC analysis on individual solutions of Lidocaine, Proranolol and Procainamide. I have been trying to use an 80:20 (MeOH:H2O), which only barely resolves the Lidocaine and Procainamide peaks, and it fails to give any peaks for Propranolol. So i just wanted to know if there is one mobile phase composition i can use that can work on all three of them? Or do i have to use a separate mobile phase for each analyte?

Regards

[danko]

It reminds med of a project I was forced to participate in some years ago in a previous employment.
A cost reduction fanatic thought that if we found a universal mobile phase that could be used with all compounds we were working on, it would have been much cheaper than preparing all those different mobile phases. Another part of the project was finding a universal column

Menlite, the best advice I can give you (others might have other ideas) is: Learn more about chromatography and you’ll find the answer to your question - and many, many more answers and ideas.

Best Regards

[Uwe Neue]

For analytes of this type, do not even dream about running without a buffer in the mobile phase.

[bisnettrj2]

James F. Wesley, Fred D. Lasky, High performance liquid chromatographic analysis of the antiarrhythmic drugs procainamide, disopyramide, quinidine, propranolol and metabolites from serum extracts, Clinical Biochemistry, Volume 14, Issue 3, The National Academy of Clinical Biochemistry (U.S.A.), June 1981, Pages 113-118, ISSN 0009-9120, DOI: 10.1016/S0009-9120(81)90225-3.
(http://www.sciencedirect.com/science/ar ... 73250a3138)

James F. Wesley, Fred D. Lasky, Simultaneous analysis of antiarrhythmic drugs and metabolites by high performance liquid chromatography: Interference studies and comparisons with other methods, Clinical Biochemistry, Volume 15, Issue 6, December 1982, Pages 284-290, ISSN 0009-9120, DOI: 10.1016/S0009-9120(82)96806-0.
(http://www.sciencedirect.com/science/ar ... 5c7a608595)

[menlite]

Thank you very much for your help