FAO Uwe de Neue: Proven ion-pair method for choline?

[chromeleon]

I need to do this by indirect UV detection. can anyone help?

T.I.A.

[chromeleon]

where have all the experts gone??

[bisnettrj2]

J Pharm Biomed Anal. 1995 Apr;13(4-5):581-8.

Ion-exchange liquid chromatography method with indirect UV detection for the assay of choline in pharmaceutical preparations.
Leroy P, Barbaras M, Colin JL, Nicolas A.

Laboratoire de Chimie Analytique, URA CNRS 597, Faculté des Sciences Pharmaceutiques et Biologiques-B.P. 403, Nancy, France.


I'm no expert, I'm just friends with Google.

[chromeleon]

thank you, but that was one of the first papers I found :lol:
it is useful but not exactly what I need

on a related note, how can I reverse the polarity of my UV detector?

[chromeleon]

baseline is all over the place
apart from filtering my buffer and controlling column temperature, any other tricks?