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- Posts: 2916
- Joined: Mon Aug 30, 2004 10:19 pm
You are correct: there can be multiple configurations of the product of the reaction of the aflatoxin epoxide with the glutathione. The literature says that there are 2 isomers, but I can visualize even more.
Anyway, at this improved pH, you are likely to have a more stable separation from the rest of the world, and all you need to do is to integrate the entire blob to get the total content of the AFB1 glutathione.
It is still possible that additional peak distortions could be accounted for from an injection of the sample in a strong solvent.
Anyway, at this improved pH, you are likely to have a more stable separation from the rest of the world, and all you need to do is to integrate the entire blob to get the total content of the AFB1 glutathione.
It is still possible that additional peak distortions could be accounted for from an injection of the sample in a strong solvent.
