by
danko » Thu Apr 01, 2010 9:27 am
Hi Rick,
It’s a kind of controversial topic here in this forum, meaning that some people are applauding it whilst other denying the existence of it, so you’ll have to make your own mind. I’ve linked to a short text as well as a video, illustrating the matter. On top of it, you can go down to the local river and watch what happens to the water when it meets a stone in its way.
Taking it back into chromatography context, the phenomenon is only achievable when the stationary phase particles are very large (just like the stones in the river – and not the sand) and the flow rate is relatively high. So, large particles (e.g. 100 µm or something like that) and a thin column (1 mm in diameter) is a good starting point to utilizing the technique.
Text:
http://www.cohesivetech.com/technologie ... /index.asp
Video:
http://www.youtube.com/watch?v=svyO5tfkYtw
Please note that I’m not working for Thermo Scientific, neither am I obliged to them in any ways.
Best Regards
