internal standard resonce ratio

[nageshiict]

I am doing bio analytical analysis by using internal standard.but i am getting more intenced peak for internal standard than analytes. For how much responce ratio, i have to use for internal standard analysis.please suggest me.

[HW Mueller]

HPLC is a method which has to be calibrated.

[JGK]

Aim for an IS concentration that will produce a response similar to that seen at the mid point of your analyte calibration range

[nageshiict]

Mr JGK
thank u for good suggetion