polystyrene divinylbenzene sulfate columns

[Noser222]

Just wondering if anyone has messed around with using ammonium (NH4+ or other amines) modifiers to get any selectivity change with carbohydrate analyses. I have seen the various salt forms from H+ to Ag+, so just wondering if it is possible to take an H+ form and treat it with an ammonium salt in the mobile phase.

[dave hometchko]

PSDVB sulfonated columns for sugars usually use sodium, calcium or lead to form chelates with sugars to obtain separation. The main eluent for these columns is water. Using acid or base will poison the packing material and destroy separation.

[tom jupille]

It's virtually impossible to change ionic form of those columns in-situ because the resins shrink/swell. As Dave pointed out, trying to do the conversion (e.g. from the H+ form to the ammonium form) will effectively kill the column.