I am attempting to calibrate the negative ion mode gain for our Thermo LTQ (see below for version info).
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It keeps failing and due to weak signal, but I can see the signal(s) for the Ultramark 1621.
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My current settings are -1500V for both MP1 & MP2 high gain, and -1400V for both MP1 & MP2 norm gain as shown below.
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Do I need to go even higher or is there something else I am missing? These multipliers are basically new.

Thanks!