Hello, we are in the process of developing a HPLC method for astaxanthin extracted from yeast and a few other carotenoids.

Here is a quick snapshot, if others are working on the same.
Thermo Vanquish @ 474nm (450,285,320 other carot)
C30 250um column @1ml/min.
5% H20 in MeOH
MTBE - 25% to 85% to 25% in 18 min.
We extract crystal with THF, then dilute with 40/60 MTBE/MeOH

The yeast yields free form asta.
Thanks all.