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HPLC PC not talking to Agilent pump or sampler

Discussions about chromatography data systems, LIMS, controllers, computer issues and related topics.

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Posting this here as it seems like best place to get a response

I run the general chemistry labs at a small college (~100-120 students per year in class) and was in 2019 gifted a second hand Agilent Series 1100 with binary pumps (G1312A), autosampler (G1329A) and DAD(G1315A). The thing has been a godsend to my teaching lab, a workhorse in therms of throuput and we published an article having general chemistry exposed to HPLC and it also is a key part of my research

Unfortunately this summer, there was a major power outage and when things came back on, the aging computer running the instrument would not boot. My campus ITS guy did a great job as he said the thermal paste in it was shot and so he replaced that, got things up and running but redone computer needed the software reinstalled as starting regular one gave BIOS error so I got the disks out and installed 2016 version of Chemstation (A02.02) that was on the disks I got with machine. HPLC parts connect via LAN and internet cable which looks in good condition.

I found the TCP that all agilent systems use and punched it in after creating an instrument and I did autoconfigure upon which it found my pumps, sampler and DAD and fetched their Model and their serial number so evidence it is talking to them?

But when I start to run the instrument (even after complete restart) it finds the detector and I can turn lamps on and off but pumps and sampler are offline with no wee button to turn on, DAD starts as Not ready but can be turned on but there seems to be no communication to Pumps or Sampler, no NOT READY, just offline. I thought through during config it talked to them as it knows their serial number but no other communication once I run the operating software. I've definitely got the standard TCP for each when I go into Open Lab control panel program and look at configurations, they seem ok with that Agilent TCP. I've not changed any cables or settings with switches on back, computer was taken apart in ITS, I just disconnected from internet cable.

Machine is not on internet as it's an older model and ITS will not allow to be networked which was fine - we got data off via USB

Oh and pumps and sampler bot make standard noises, arm swing when turned on so they do their own warm-up ok, just will not talk to the PC it seems

any thoughts or advice welcome
You could try a cold start procedure maybe? I remember doing that a few years ago for a similar issue after a power outage and it fixed it for me;

https://community.agilent.com/knowledge ... -procedure

I run the general chemistry labs at a small college (~100-120 students per year in class) and was in 2019 gifted a second hand Agilent Series 1100 with binary pumps (G1312A), autosampler (G1329A) and DAD(G1315A).

...redone computer needed the software reinstalled as starting regular one gave BIOS error so I got the disks out and installed 2016 version of Chemstation (A02.02) that was on the disks I got with machine. HPLC parts connect via LAN and internet cable which looks in good condition.
GraemeW - We had Agilent 1100 with similar components (before 2016 though) and ours ran on "A"version software, so that's all I'm familiar with. I've never heard of "A02.02", wonder if that's a typo?

Anyway, because you're at a college, I would like to gift you a version of "A" software if you would like. I can supply A.06 through A.10, and you'd have to supply your E-mail address.
3 posts Page 1 of 1

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